ThermaStop is a proprietary additive for use in reactions involving any thermostable DNA polymerase and is designed to dramatically improve reaction specificity and yield for cleaner, robust reactions. ThermaStop interacts with DNA polymerase at temperatures below 50ºC to provide reversible inhibition of enzyme activity. ThermaStop also inhibits DNA polymerase activity following PCR, thereby providing a "cold-stop" that can improve results from subsequent product analysis.
One unit of ThermaStop is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 µl.
A single tube of dried ThermaStop reagent. Does not contain magnesium, dNTPs, or buffer components.