ThermaStopRT

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ThermaStop-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcription-PCR assays containing gene-specific primers.  ThermaStop-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors that lead to non-specific products.  By eliminating these errors, ThermaStop-RT  can significantly improve detection sensitivity and specific product yield.  One unit of ThermaStop-RT  is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase and one unit of hot-start Taq Polymerase in a volume of 20 μL and a reverse transcription temperature of 50oC.  ThermaStop-RT  should not be used in combination with ThermaStop or ThermaGo.

 

A single tube of dried ThermaStop-RT reagent. Does not contain magnesium, dNTPs, or buffer components.

 

 

 

ThermaStop-RT Data Sheet

THE FUTURE OF PCR

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THE FUTURE OF PCR