ThermaStop-RT is a proprietary additive for One-Step, or Two-Step Reverse Transcription-PCR assays containing gene-specific primers. ThermaStop-RT interacts with the reverse transcriptase at low temperatures to reduce priming errors that lead to non-specific products. By eliminating these errors, ThermaStop-RT can significantly improve detection sensitivity and specific product yield. One unit of ThermaStop-RT is defined as the amount required for optimal performance in a one-step RT-PCR containing 50 units of reverse transcriptase and one unit of hot-start Taq Polymerase in a volume of 20 μL and a reverse transcription temperature of 50oC. ThermaStop-RT should not be used in combination with ThermaStop or ThermaGo.
A single tube of dried ThermaStop-RT reagent. Does not contain magnesium, dNTPs, or buffer components.