ThermaGo acts at elevated temperatures during annealing and elongation to prevent scatter due to unwanted chimera and mis-priming product formation.
ThermaGo interacts with DNA polymerase during the annealing and extension steps of PCR to improve amplification specificity and prevent PCR artifacts.
One unit of ThermaGo is defined as the amount
required for maximum improvements in specificity, yield, and reproducibility in amplification reactions containing 1 unit of Taq DNA polymerase in a volume of 25 µl. Some adjustment of ThermaGo to Taq ratio maybe needed to obtain optimal results.
A single tube of dried ThermaGo reagent. Does not contain magnesium, dNTPs, or buffer components.